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Cat No | ABQ-ES96-IL1Bhu |
Size | Rate |
96T | $450 |
48T | $230 |
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This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human IL-1β.Standards or samples containing Human IL-1β are added to the plate and reacted with capture antibody. A second anti-Human IL-1β antibody labeled biotin is then added and binds to Human IL-1β captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Human IL-1β-biotin labeled antibody- SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human IL-1β in the samples is then calculated from the OD value byestablishing a standard curve
Product name | “Quanticon” Human IL-1β(Interleukin 1
Beta) ELISA Kit |
Cat no | ABQ-ES96-IL1Bhu |
Applications | ELISA |
Reactivity | Human |
Standard Range | 7.82-500pg/mL |
Sensitivity | 4.69 pg/mL. |
Sample Type | serum, plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 ul |
Alternative | IL1B, IL1-BETA, IL1F2, catabolin |
Assay Type | Sandwich |
Assay Duration | 3.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item | Intra-assay Precision | Intra-assay Precision |
Sample number | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Matrix type | Recovery Range (%) | Average (%) |
Serum (n=5) | 81-95 | 88 |
EDTA plasma (n=5) | 80-95 | 86 |
Cell culture media (n=5) | 83-97 | 90 |
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