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Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human IL-1β.Standards or samples containing Human IL-1β are added to the plate and reacted with capture antibody. A second anti-Human IL-1β antibody labeled biotin is then added and binds to Human IL-1β captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Human IL-1β-biotin labeled antibody- SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human IL-1β in the samples is then calculated from the OD value byestablishing a standard curve

Specification

Product name “Quanticon” Human IL-1β(Interleukin 1

Beta) ELISA Kit

Cat no ABQ-ES96-IL1Bhu
Applications ELISA
Reactivity Human
Standard Range 7.82-500pg/mL
Sensitivity 4.69 pg/mL.
Sample Type serum, plasma, Tissue homogenate and Other biological samples
Sample Volume 100 ul
Alternative IL1B, IL1-BETA, IL1F2, catabolin
Assay Type Sandwich
Assay Duration 3.5H
Detection Wavelength 450nm
Storage 2-8oC

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item Intra-assay Precision Intra-assay Precision
Sample number 3 3
Replicate 9 18
CV(%) 5 8

 

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Matrix type Recovery Range (%) Average (%)
Serum (n=5) 81-95 88
EDTA plasma (n=5) 80-95 86
Cell culture media (n=5) 83-97 90
“Quanticon” Human IL-1β(...

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