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Cat. No: | ABQ-ES96-TNFAhu |
Size | Rate |
96T | $450 |
48T | $230 |
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Principle
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human TNF-α. Standards or samples containing Human TNF-α are added to the plate and reacted with capture antibody. A second anti-Human TNF-α antibody labelled biotin is then added and binds to Human TNF-α captured on the plate. After that, Streptavidin- Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human TNF-α-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human TNF-α in the samples is then calculated from the OD value by establishing a standard curve.
Specification
Product name | QuanticonTM Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit |
Cat no | ABQ-ES96-TNFAhu |
Applications | ELISA |
Reactivity | Human |
Standard Range | 7.81-500pg/mL |
Sensitivity | 3.91 pg/mL. |
Sample Type | Serum, plasma and other biological fluids, Sample |
Sample Volume | 100 ul |
Alternative | DIF, TNF-alpha, TNFA, TNFSF2 |
Assay Type | Sandwich |
Assay Duration | 3.5H |
Detection Wavelength | 450nm |
Storage | If the kit is not open Store whole kit: 2-8°C (short time storage valid for 6 months) ; -20°C (long- term storage , valid for 1 year. |
Precision
Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.
Item | Intra-assay Precision | Intra-assay Precision |
Sample number | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Matrix type | Recovery Range (%) | Average (%) |
Serum (n=5) | 80-90 | 85 |
EDTA plasma (n=5) | 84-106 | 100 |
Cell culture media (n=5) | 87-93 | 90 |
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