“Quanticon” Human NAGase(N-Acetyl Beta-D-Glucosaminidase) ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human NAGase. Standards or samples containing Human NAGase are added to the plate and reacted with capture antibody. A second anti-Human NAGase antibody labeled biotin is then added and binds to Human NAGase captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human NAGase-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human NAGase in the samples is then calculated from the OD value by establishing a standard curve

Specification

Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.

Rate of recovery

Three matrices listed below were spiked with certain level of Human NAGase, The recovery rates of Human NAGase were calculated by comparing the measured value to the expected amount of Human NAGase in samples.