| RK004-0100 | RScript™ miRNA cDNA Synthesis Kit (Stem-loop) |
| Size | 100 rxns |
| Description | This kit is developed based on RScript II reverse transcriptase, which uses a reverse transcription primer with a stem-loop structure binding to the 3′ end of the miRNA molecule. Under the action of RScript II reverse transcriptase, the artificially elongated miRNA first-strand cDNA is obtained. The recommended general stem-loop sequence is 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAC-3′; other stem-loop sequences can also be selected according to experimental needs. Typically, reverse transcription primers only need to add 6 reverse, complementary bases at the 3′ end of the miRNA to the stem-loop sequence. This method is highly specific and only conducts reverse transcription on the mature miRNAs without being interfered by its precursors. The miRNAs with highly homologous sequences can be accurately distinguished. The reverse transcription products obtained with this product can be directly used for subsequent quantitative detection by dye or probe methods.
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