“Quanticon” Canine Collagen type 3 ELISA Kit

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. The micro plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT/pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotinconjugated antibody specific to Human pMAPT/pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pMAPT/pTAU, biotinconjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm±3nm. The concentration of pMAPT/pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

 Intra-Assay: CV<8%

 Inter-Assay: CV<10%