“Quanticon” Canine Collagen type 1 ELISA Kit

Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine COL1 antibody. COL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine COL1 Antibody is added and binds to COL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated COL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine COL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Specification

Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.The results showed that the coefficient of variation of the kits was less than 10%, which met the precision quality control standard.

Intra-Assay: CV<5%

Inter-Assay: CV<8%