Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IL1B antibody. IL1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IL1B Antibody is added and binds to IL1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IL1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon“ Porcine Interleukin 1 Beta, IL-1B ELISA Kit |
| Cat no | ABQ-ES96-IL1Bpr |
| Applications | ELISA |
| Reactivity | Porcine |
| Standard Range | 1-300ng/L |
| Sensitivity | 0.46ng/L |
| Sample Type | Serum, plasma, Tissue, Urine and Cell culture supernatant |
| Sample Volume | 100 μL |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
PRECISION
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra- assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%.
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