“Quanticon” E2 (Estradiol) ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Porcine TNF-α. Standards or samples containing Porcine TNF-α are added to the plate and reacted with capture antibody. A second anti- Porcine TNF-α antibody labeled biotin is then added and binds to Porcine TNF-α captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody- Porcine TNF-α-biotin labeled antibody-SAHRP. And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Porcine TNF-α in the samples is then calculated from the OD value by establishing a standard curve.

Specification

PRECISION

Mean coefficient of variation for Intra- Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively .The results showed that the

coefficient of variation of the kits was less than 10%, which met the precision quality control standard..