Principle
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-PEG IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-PEG IgG in the sample by plotting standard curve.
Specification
| Product name | “Quanticon”IGMRabbit anti-PEG(Polyethylene glycol) IgM ELISA Kit |
| Cataloge no | ABQ-ES96-IGMrb |
| Applications | ELISA |
| Reactivity | Rabbit |
| Standard Range | 0.625-40ng/ml |
| Sensitivity | 0.375ng/ml |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Sample Volume | 100 μL |
| Assay Type | Sandwich |
| Assay Duration | 3 H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
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