| Product Name | CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit |
| Catalogue No | KTB1010 |
| Applications Notes | Abbkine CheKine™ Micro Coenzyme Ⅱ NADP(H) Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. |
| Kit components | • Assay Buffer
• Glucose (1 M) • WST-8 Solution • Enhancer Solution • NADP Cycling Enzyme Mix • NADPH standard (10 mM) • NADP Extraction Buffer • NADPH Extraction Buffer |
| Features & Benefits | • Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay. |
| Storage | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Formulations | Liquid solution ready to use |
| Background | Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues. |
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