| Product Name | CheKine™ Micro Glucose Oxidase Activity (GOD) Assay Kit |
| Catalogue No | KTB1310 |
| Applications Notes | CheKine™ Micro Glucose Oxidase Activity Assay Kit provides a simple and easy colorimetric assay for detecting Glucose Oxidase Activity in biological samples such as in serum or plasma, cells, urine, other body fluid, food, growth medium. In this assay, GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with chromogen in an acid condition, produce a product that can be measured at OD 580 nm. Therefore, the glucose oxidase activity present in the sample is proportional to the signal obtained. |
| Kit components | • Assay Buffer (20x)
• Glucose (0.2 M) •H2O2 Standard (0.88 M) • Chromogen • Glucose Oxidase (control) |
| Features & Benefits | • Optimized procedure for detecting Glucose Oxidase Activity in biological samples such as in serum or plasma, cells, urine, other body fluid, food, growth medium.
• Simple and easy colorimetric assay. • Quick and convenient. |
| Storage | Storage at –20°C and keep from light. Kit has a storage time of 12 months from receipt. |
| Shipping | Gel pack with blue ice. |
| Formulations | Liquid solution ready to use |
| Background | The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of β-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. This enzyme is produced by certain species of insects, fungi, and bacteria. Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose. |
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