| Product Name | CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit |
| Catalogue No | KTB1640 |
| Applications Notes | GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity. |
| Kit components | • Assay Buffer
• Substrate • GR • H2O2 |
| Features & Benefits | • Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Storage | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Formulations | Liquid solution ready to use |
| Background | GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body’s ability to resist radiation. |
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