| Product Name | CheKine™ Micro Hydrogen Peroxide (H2O2) Assay Kit |
| Catalogue No | KTB1041 |
| Alternative | Modified Basal Medium 5A |
| Applications notes | CheKine™ Micro Hydrogen Peroxide Assay Kit provides a simple and easy colorimetric assay for measuring hydrogen peroxide in serum, plasma, cell culture supernatants, tissue/cell lysates and other biological fluids. In the assay, H2O2 oxidize ferrous to ferric ion, and xylenol orange binds ferric ion with high selectivity to form a colored (purple) product that can be measured by colorimetric method at OD 580 nm. The Hydrogen Peroxide present in the sample is proportional to the signal obtained. |
| Kit components | • Reaction Buffer • H2O2 Standard (1M) • Assay Buffer (10X) |
| Features & Benefits | • Determination of Hydrogen Peroxide in serum, plasma, plant or animal tissue samples, urine and other biological fluids. • Simple procedures, fase and convenient, and more stable. |
| Usage notes | • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
• Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month. |
| Storage | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Background | Hydrogen Peroxide (H2O2), a reactive oxygen metabolic by-product, which serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neurodegenerative diseases, Down’s syndrome and immune system diseases. |
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