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Product Name | DMEM/F12 Medium |
Catalogue No | BMC1015 |
Application | Cell culture |
Applicable samples | Cells and Tissues |
Storage | Stored at 4℃ for 12 months, protected from light |
Shipping | Gel pack with blue ice. |
Formulation | Liquid solution, ,ready to use |
Assay Principle | DMEM/F-12 Medium (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEMmediumandHam’sF-12 medium, based on DMEM medium. F-12 was added to enrich the nutrient content, containing a variety of trace elements.Dulbecco Modified Eagle Medium (DMEM) is a modified Eagle Basic Medium (BME) with four times the amino acid and vitamin concentration of BME. Ham’s F-12, based on Ham’s F-10 medium, significantly increased the concentration of choline, inositol, putputylamine and several amino acids. DMEM/F-12 medium is widely used to support the growth of a variety of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. At the same time, DMEM/F12mediumis often used as the basis for the development of serum-free medium, and it is also suitable for the culture of mammalian cells with low serum content and clone density. DMEM/F12 Medium contains amino acids, vitamins, inorganic salts and other components required for multi-type cell culture, but does not contain lipids or any growth factors, so the product should be used with serum or without serum additives. This product is filtered with 0.22 μm filter membrane to remove bacteria, without high temperature and high pressure sterilization, less nutrient loss, ready to open the bottle; To provide a variety of component combinations of cell media to meet various needs. It is suitable for a variety of mammalian cell culture |
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