Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CYP27B1 antibody. CYP27B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP27B1 Antibody is added and binds to CYP27B1 in the sample. Then Streptavidin- HRP is added and binds to the Biotinylated CYP27B1antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CYP27B1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon” Human 25-hydroxyvitamin D-1 alpha hydroxylase,mitochondrial, ELISA Kit |
| Cat no | ABQ-ES-25OHD1Ahu |
| Applications | ELISA |
| Reactivity | Human |
| Standard Range | 20-3800ng/L |
| Sensitivity | 9.47ng/L |
| Sample Type | Serum, plasma, Cell culture supernatant, and other biological fluid. |
| Alternative | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D (3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
Precision
Intra-Assay Precision (Precision within an assay) three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
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