“Quanticon” Human IL-1β(Interleukin 1 Beta) ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human IL-1β.Standards or samples containing Human IL-1β are added to the plate and reacted with capture antibody. A second anti-Human IL-1β antibody labeled biotin is then added and binds to Human IL-1β captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Human IL-1β-biotin labeled antibody- SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human IL-1β in the samples is then calculated from the OD value byestablishing a standard curve

Specification

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.