“Quanticon” Human OXA (Orexin A) ELISA Kit

Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated withHuman OXA. During the reaction,Human OXA in the sample or standard competes with a fixed amount of Human OXA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OXA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human OXA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Specification

Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.

Rate of recovery

Three matrices listed below were spiked with certain level of Human OXA, The recovery rates of Human OXA were calculated by comparing the measured value to the expected amount of Human OXA in samples.