Quanticon Human PORCN(Probable protein-cysteine N-palmitoyltransferase porcupine) ELISA Kit

Principle

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PORCN antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PORCN antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PORCN conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PORCN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional.

Specification

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively

Intra-Assay: CV<5%

Inter-Assay: CV<8%