“Quanticon” PGE2 (Prostaglandin E2) ELISA Kit

Principle

This assay is based on the competitive binding technique. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction, PGE2 in samples or Standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE2. Following a wash to remove excess conjugate and unbound sample. Then Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Substrate solution is added to the wells. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±3nm. The intensity of the color is inversely proportional to the concentration of PGE2 in the sample.

Specification

Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively. The results showed that the coefficient of variation of the kits was less than 10%, which met the precision quality control standard.

Intra-Assay: CV<5%

Inter-Assay: CV<8%