Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine Estrogen antibody. Estrogen present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine Estrogen Antibody is added and binds to Estrogen in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Estrogen antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine Estrogen. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon” Porcine Estrogen, ER ELISA KIT |
| Cat no | ABQ-ES96-ERpr |
| Applications | ELISA |
| Reactivity | Porcine |
| Standard Range | 6.25-400ng/L |
| Sensitivity | 3.06ng/L |
| Sample Type | Serum, plasma , Cell culture supernatant and other biological fluids, Sample |
| Sample Volume | 50 ul |
| Alternative | E ; Estrogen |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month. |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
CV(%) = SD/mean x 100
- Intra-Assay: CV<8%
- Inter-Assay: CV<10%
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