“Quanticon”LPS(Lipopolysaccharides) ELISA Kit

Principle

This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti LPS antibody. Sample or standard completely mixes with fixed amount of biotin-labeled LPS working solution and also add into each well. LPS in the sample or standard competes with biotin-labeled LPS to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of LPS in the sample.

Specification