Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine TGF-β1 antibody. TGF-β1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine TGF-β1 Antibody is added and binds to TGF-β1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TGF-β1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine TGF-β1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon” Canine Transforming Growth factor β1 ELISA Kit |
| Cat no | ABQ-ES96- TGFB1cn |
| Applications | ELISA |
| Reactivity | Canine |
| Standard Range | 0.3-90ng/ml |
| Sensitivity | 0.16ng/ml |
| Sample Type | Serum, plasma, Urine, Cell culture supernatant and Tissue |
| Sample Volume | 50 μL |
| Alternative | Latency-associated peptide and LAP |
| Assay Type | Sandwich |
| Assay Duration | 1.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
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