“Quanticon” GSH(Glutathione) ELISA Kit

Principle

This ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GSH. During the reaction,GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Specification

 Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively

Rate of recovery

Three matrices listed below were spiked with certain level of GSH, The recovery rates of GSH were calculated by comparing the measured value to the expected amount of GSH in samples