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Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Huaman Maresin 1. Standards or samples containing Huaman Maresin 1 are added to the plate and reacted with capture antibody. A second anti-Huaman Maresin 1 antibody labeled biotin is then added and binds to Huaman Maresin 1 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Huaman Maresin 1-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Huaman Maresin 1 in the samples is then calculated from the OD value by establishing a standard curve.

Specification

Product name “Quanticon Human Maresin 1 ELISA Kit
Cat no ABQ-ES96-MAR1hu
Applications ELISA
Reactivity Human
Standard Range 15.63-1000pg/mL..
Sensitivity 9.38 pg/mL.
Sample Type Serum, plasma, Tissue homogenates, Cell lysates, Cell culture supernatant and other biological fluids
Sample Volume 100 μL
Assay Type Sandwich
Assay Duration 3.5H
Detection Wavelength 450nm
Storage 2-8oC

 

PRECISION

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.The results showed that the coefficient of variation of the kits was less than 10%, which met the precision quality control standard.

Intra-Assay: CV<5%

Inter-Assay: CV<8%

“Quanticon” Human ...

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