“Quanticon” Porcine IL-12(Interleukin 12)ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle.The microtiter plate strips has been pre-coated with an affinity purified antibody to Porcine IL-12. Standards or samples containing Porcine IL-12 are added to the plate and reacted with capture antibody. A second anti-Porcine IL-12 antibody labeled biotin is then added and binds to Porcine IL-12 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SAHRP) is added to form a sandwich complex of solid phase antibody-Porcine IL-12-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Porcine IL-12 in the samples is then calculated from the OD value by establishing a standard curve.

Specification

PRECISION

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.The results showed that the coefficient of variation of the kits was less than 10%, which met the precision quality control standard.