| RK005-0100 | RScript™ miRNA cDNA Synthesis Kit (Poly-A) |
| Size | 100 rxns |
| Description | This kit is a special kit for the first-strand cDNA synthesis by poly(A) microRNA (miRNA), which contains all the raw materials for the miRNA -poly A-tail and reverse transcription reactions. Through careful optimization, it ensures that the poly(A) modification process at the 3′ end of miRNA and reverse transcription process can be carried out efficiently at the same time. The RScript II miRNA-A enzyme mix contains the poly(A) polymerase (PAP) and RScript II Reverse Transcriptase. The high efficiency rendered by the PAP-poly A tail can specifically recognize the single-stranded RNA and effectively avoid the reverse transcription reaction of miRNA precursors with the double-stranded or stem-loop structures. RScript II Reverse Transcriptase has been engineered to lose the RNase H activity and increase the affinity with RNA, resulting in a significant increase in the reverse transcription efficiency and sensitivity of miRNAs. The reverse transcription products obtained with this product can be directly used for subsequent quantitative detection by dye or probe methods.
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