TRUEzol Reagent

ClearBand TRUEzol LS Reagent is a ready-to-use reagent composed of phenol and a mixture of other components for the isolation of high-quality total RNA from various liquid biological materials of human, animal, plant, yeast, bacterial, and viral origin. ClearBand  TRUEzol LS Reagent is not suitable for use with whole blood.

Biological materials are homogenized or lysed in ClearBand  TRUEzol LS Reagent and then separated into three phases: a clear upper aqueous phase with the RNA, a pink lower organic phase and an interphase, containing DNA and protein. RNA is purified by precipitation with isopropyl alcohol and then washed to remove impurities.

Purified RNA can be effectively used in routine downstream applications including cDNA synthesis, northern blotting, differential display, primer extension, and mRNA selection