| Product Name | CheKine™ Micro Total Antioxidant Capacity (TAC) Assay Kit |
| Catalogue No | KTB1500 |
| Applications Notes | CheKine™ Micro Total Antioxidant Capacity (TAC) Assay Kit provides a simple and easy colorimetric assay for measuring Total Antioxidant Capacity in serum, plasma, cell culture supernatants, urine, tissue/ cell lysates and other biological fluids. In this assay, Fe3+-TPTZ is reduced by antioxidant to Fe2+-TPTZ. The enzyme catalyzed reaction products Fe2+-TPTZ can be measured at a colorimetric readout at 593 nm. |
| Kit components | • Assay Buffer (10X)
• Substrate Diluent • Substrate • Reaction Buffer • ascorbic acid (positive control) |
| Features & Benefits | • Determination of Total Antioxidant Capacity in serum, plasma, cell/tissue samples, urine and bacteria samples.
• Using a stronger antioxidant, sascorbic acid as positive control, instead of the frequently-used Trolox. • The reaction is carried out under acidic conditions, which can inhibit some endogenous interference factors. |
| Storage | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Formulations | Liquid solution ready to use |
| Background | Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.) |
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