Product Name

CheKine™ Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Catalogue No

KTB1410

Applications Notes

The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37°C and pH7.4 to produce glutamic acid and pyruvate; pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.

Kit components

• Extraction Buffer

• Reagent I

• Reagent II

• Reagent III

• Standard

Features & Benefits 

• Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.

• Detailed sample preparation and result calculation methods are provided.

• Simple operation steps.

• Standard and standard curve line are provided.

Storage

Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping

Gel pack with blue ice.

Background

Alanine Aminotransferase (ALT/GPT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, ALT has the highest content in cardiomyocytes, and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice.