CheKine™ Micro Phosphoenolpyruvate Carboxykinase（PEPCK) Activity Assay Kit
CheKine™ Micro Phosphoenolpyruvate Carboxykinase（PEPCK) Activity Assay Kit
CheKine™ Micro Phosphoenolpyruvate Carboxykinase（PEPCK) Activity Assay Kit
Applications Notes
CheKine™ Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit provides a simple, convenient and rapid method for PEPCK activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma) and other samples. The principle is that PEPCK catalyzes the oxidation of oxaloacetate to produce phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to produce NAD+ in turn, The rate of NADH decline was measured at 340 nm, which reflects PEPCK activity.
Product Name | CheKine™ Micro Phosphoenolpyruvate Carboxykinase（PEPCK) Activity Assay Kit |
Catalogue No | KTB1126 |
Kit components | •Extraction Buffer •ReagentⅠ •ReagentⅡ •ReagentⅢ •Reagent IV •Reagent V |
Features & Benefits | • Determination of PEPCK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. |
Storage | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Formulations | Liquid solution ready to use |
Background | PEPCK (EC 4.1.1.32) is widely found in animals, plants, microorganisms and cells and catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, a key enzyme in the regulation of gluconeogenic pathways. |
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