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Product Name | MEM,with NEAA |
Catalogue No | BMC1012 |
Alternative | Minimum Essential Medium、minimal essential medium, or low limit Eagle medium |
Application | IHC, ICC, IF, ELISA, BL |
Storage | Stored at 4℃ for 12 months, protected from light |
Shipping | Gel pack with blue ice. |
Formulation | Liquid solution, ,ready to use |
Background | MEM (Minimum Essential Medium), also known as the minimum essential medium, minimum essential medium or low limit Eagle medium, developed by Harry Eagle on the basis of the Eagle Basic Medium (BEM), is the most basic and most applicable medium. It is one of the most commonly used media for animal cell culture. MEM contains only 12 essential amino acids, glutamine and 8 vitamins, the composition is simple, mainly used for the culture of adherent cells, the formula can also be used for other types of cell culture after modification. MEM, With NEAA, which is based on MEM with the addition of L-alanine, L-glutamic acid, L-asparagine, L-aspartate, L-proline, L-serine and glycine 7 kinds of NEAA, can reduce the side effects of cell production of non-essential amino acids during cell culture and effectively promote cell proliferation and metabolism. MEM, With NEAA contains amino acids, vitamins, inorganic salts and other components required for multi-type cell culture, but does not contain proteins, lipids or any growth factors, so the product should be used with serum or without serum additives. This product is filtered with 0.22 μm filter membrane to remove bacteria, without high temperature and high pressure sterilization, less nutrient loss, ready to open the bottle; To provide a variety of component combinations of cell media to meet various needs; It is suitable for a variety of mammalian cell cultures, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts and primary rat astrocytes. |
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