Quanticon Canine TNF-α(TumorNecrosis Factor Alpha) ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Canine TNF-α. Standards or samples containing Canine TNF-α are added to the plate and reacted with capture antibody. A second anti-Canine TNF-α antibody labeled biotin is then added and binds to Canine TNF-α captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Canine TNF-α-biotin labelled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Canine TNF-α in the samples is then calculated from the OD value by establishing a standard curve.

Specification

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-Assay: CV<5%

Inter-Assay: CV<8%