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Cat No | ABQ-ES96-IL4hu |
Size | Rate |
96T | $450 |
48T | $230 |
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-4. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name | “Quanticon” Human IL-4(Interleukin 4) ELISA Kit |
Cat no | ABQ-ES96-IL4hu |
Applications | ELISA |
Reactivity | Human |
Standard Range | 31.25-2000pg/mL. |
Sensitivity | 18.75 pg/mL. |
Sample Type | serum, plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 ul |
Alternative | IL4, BCGF-1, BCGF1, BSF-1, BSF1 |
Assay Type | Sandwich |
Assay Duration | 3.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item | Intra-assay Precision | Intra-assay Precision |
Sample number | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Matrix type | Recovery Range (%) | Average (%) |
Serum (n=5) | 83-96 | 89 |
EDTA plasma (n=5) | 92-107 | 99 |
Cell culture media (n=5) | 92-105 | 98 |
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