Quanticon Human Irisin ELISA

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human Irisin. Standards or samples containing Human Irisin are added to the plate and reacted with capture antibody. A second anti-Human Irisin antibody labeled biotin is then added and binds to Human Irisin captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human Irisin-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human Irisin in the samples is then calculated from the OD value by establishing a standard curve.

Specification

PRECISION

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter- Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess Inter-Assay precision.

CV (%)= SD/mean x 100

Intra-Assay: CV<5%

Inter- Assay: CV<8%