Principle
Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT1 in samples. An antibody specific for Human WNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated WNT1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of WNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specification
| Product name | Quanticon Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit |
| Cat no | ABQ-ES96-WNT1hu |
| Applications | ELISA |
| Reactivity | Human |
| Standard Range | 7.5 pg/mL-120 pg/mL |
| Sensitivity | 0.5 pg/mL |
| Sample Type | Serum, plasma, Tissue homogenates, Cell culture supernatant and other biological fluids |
| Sample Volume | 50 μL |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)＜9%.
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)＜11%.
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