“Quanticon” Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Principle

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Mouse TNF-α. Standards or samples containing Mouse TNF-α are added to the plate and reacted with capture antibody. A second anti-Mouse TNF-α antibody labeled biotin is then added and binds to Mouse TNF-α captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Mouse TNF-α-biotin labeled antibody- SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Mouse TNF-α in the samples is then calculated from the OD value by establishing a standard curve.

Specification

Precision

Mean coefficient of variation for Intra-Assay and Inter-Assay: 3 samples with low, middle and high level concentration were tested for repeat multiple times, respectively.

Rate of recovery

Three matrices listed below were spiked with certain level of Mouse TNF-α, The recovery rates of Mouse TNF-α were calculated by comparing the measured value to the expected amount of Mouse TNF-α in samples