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Principle

This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of PGFM in the sample. The concentration of PGFM in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Specification

Product name “Quanticon” Porcine 13,14-dihydro-15-keto-prostaglandin F2 alpha ELISA Kit
Cat no ABQ-EC-PGFMpr
Applications ELISA
Reactivity Porcine
Standard Range 18.75-1200ng/L
Sensitivity 8.85ng/L
Sample Type Serum, plasma, Cell culture supernatant, and other biological fluid.
Alternative PGFM, 13,14-Dihydro-15-keto-PGF2α, 13,14-DHK-PGF2 alpha
Assay Type competitive
Assay Duration 3.5H
Detection Wavelength 450nm
Storage 2-8oC

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV< 10%

Inter-Assay: CV< 12%

“Quanticon” Porcine 13,14-dihy...

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