“Quanticon” Porcine Progesterone ELISA Kit
Cat. No: ABQ-EC96-PROGpo
Size Rate
96T $450
48T $230
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Principle
This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). Add samples to the pre-coated plate.Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigento bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stoppedby addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of PROG in the sample. The concentration of PROG in the sample is then determined by comparing the O.D. of the samples to the standard curve.
Specification
| Product name | “Quanticon” Porcine Progesterone ELISA Kit |
| Cat no | Cat. No: ABQ-EC96-PROGpo |
| Applications | ELISA |
| Reactivity | Porcine |
| Standard Range | 0.2-60ng/ml |
| Sensitivity | 0.098ng/ml |
| Sample Type | Serum, plasma , Cell culture supernatant and other biological fluids, Sample |
| Sample Volume | 50 ul |
| Alternative | Pg, Progesterone |
| Assay Type | competitive |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month. |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
- Intra-Assay: CV< 10%
- Inter-Assay: CV< 12%
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