Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CD33 antibody. CD33 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CD33 Antibody is added and binds to CD33 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD33 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CD33. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon” Rat Siglec3/CD33 ELISA Kit |
| Cat no | ABQ-ES96-CD33rt |
| Applications | ELISA |
| Reactivity | Rat |
| Standard Range | 50-3200ng/L |
| Sensitivity | 27.85ng/L |
| Sample Type | Serum, plasma, Cell culture supernatant, and other biological fluid. |
| Alternative | SIGLEC3, CD33, gp67, CD33 antigen, CD33 antigen, gp67, CD33 molecule, FLJ00391, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC-3, Siglec-3, SIGLEC3gp67 |
| Assay Type | Sandwich |
| Assay Duration | 1.5 H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
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“Quanticon” Rat Siglec3/CD33 E...
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