“Quanticon”Bovine serum albumin(BSA) ELISA Kit

Principle

This kit is based on Competitive-ELISA detection method and takes 1h assay time. The microplate provided in this kit has been precoated with anti BSA antibody. Sample or standard completely mixes with fixed amount of HRP labeled BSA working solution and also add into each well. BSA in the sample or standard competes with biotin-labeled BSA to bind with antibody precoated on the microplate. Free components are washed off. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of BSA in the sample.

Specification