Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse mTORC1 antibody. mTORC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse mTORC1 Antibody is added and binds to mTORC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated mTORC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse mTORC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | “Quanticon” Mouse mTOR Complex 1 ELISA Kit |
| Cat no | ABQ-ES96-MTORC1ms |
| Applications | ELISA |
| Reactivity | Mouse |
| Standard Range | 62.5-4000ng/L |
| Sensitivity | 31.53ng/L |
| Sample Type | Serum, plasma, Cell culture supernatant, and other biological fluid. |
| Alternative | mTOR Complex 1 |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC |
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“Quanticon” Mouse mTOR Complex...
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