Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MCH antibody. MCH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MCH Antibody is added and binds to MCH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MCH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MCH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Specification
| Product name | QuanticonTM Rat Melanin-concentrating hormone ELISA Kit |
| SKU | ABQ-ES96-MCHrt |
| Applications | ELISA |
| Reactivity | RAT |
| Sensitivity | 0.83pg/ml |
| Detection Range | 1.88-120pg/ml |
| Sample Type | Serum, Plasma, Urine/Ascites/ Cerebrospinal fluid, Cell culture supernatant and Tissue |
| Sample Volume | 50 μL |
| Assay Type | Sandwich |
| Detection Wavelength | 450nm |
| Storage | 2-8oC/-20 oC |
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