Principle of the Procedure

Alkaline Phosphatase (AKP/ALP) is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP/ALP is widely distributed in various organs of the human body, mainly the liver. In an alkaline environment, AKP/ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate can reflect AKP/ALP activity.