Principle of the Procedure

Glutamic-pyruvic Transaminase(GPT)/Alanine Aminotransferase(ALT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum GPT/ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, GPT/ALT has the highest content in cardiomyocytes, and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. This kit provides a simple method for detecting GPT/ALT activity in a variety of biological samples. In the assay, GPT/ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37℃ and pH7.4 to produce Glutamic Acid and Pyruvate; Pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate Phenylhydrazone, which appears brownish red under Alkaline conditions and has a characteristic absorption peak at 505nm. The rate of Pyruvate Phenylhydrazone increase at 505 nm can reflect GPT/ALT activity.