Principle of the Procedure

Glucose (C6H12O6, FW: 180.16) is the primary biological fuel source used to generate the universal energy molecule, ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameter for many metabolic disorders. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulfonylureas in type 2 diabetics. This kit can detect samples of serum, plasma, urine, other body fluid, food, growth medium, etc. The principle is that the improved o-Toluidine method utilizes a specific color reaction with glucose. The absorbance at 630 nm is directly proportional to glucose concentration in the sample.