Principle of the Procedure

Glycogen is a polymer polysaccharide composed of glucose. It is one of the main storage forms of glucose. It is mainly stored in liver and muscle as reserve energy, which is called liver glycogen and muscle glycogen, respectively. Liver glycogen can regulate the concentration of blood glucose. When blood glucose rises, glycogen can be synthesized in the liver. When blood glucose decreases, liver glucose principle decomposes into glucose to supplement blood glucose. Therefore, liver glycogen is very important to maintain the relative balance of blood glucose. Muscle glycogen is the storage form of glucose in muscle. When strenuous exercise consumes a large amount of blood glucose, muscle glycogen cannot be decomposed directly into blood glucose. It was decomposed to produce lactic acid and circulate to the liver with blood. It is transformed into liver glycogen and glucose by gluconeogenesis.Glycogen Assay kit can detect glycogen concentration in animal tissues, bacteria and cells. The principle of kit is strong alkaline extract glycogen. Under strong acidic conditions, blue compounds with anthrone chromogenic agent with characteristic absorption peak at 620 nm. In a certain concentration range, glycogen concentration is linearly related to 620 nm absorbance. According to the standard curve, the glycogen concentration in the sample can be calculated.