Principle of the Procedure

Glutathione S-transferases (GST) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble. The glutathione conjugates are metabolized further to mercapturic acid and then excreted. GST has the activity of GSH-Px, also called non-Se GSH-Px, which has the function of repairing oxidative damage of macromolecule such as DNA, protein and so on. GST catalyzed reaction decreased GSH content, but did not increase GSSG content. Based on their sequence homology, substrate specificity and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes. These classes are comprised of both cytosolic and microsomal enzymes.Glutathione S-Transferase (GST) Assay Kit provides a simple method for detecting activity of GST in a variety of biological samples such as Serum, Plasma, Animal Tissues, Cells and Bacteria. GST catalyzes the binding of GSH to CDNB. The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample.