Principle of the Procedure

Low Density Lipoprotein Cholesterol (LDL-C) as one of the serum proteins, mainly synthesized by the liver. It’s a lipoprotein particle that carries cholesterol into peripheral tissue cells. LDL can enter the cells of the artery wall and carry cholesterol into it. It can also be oxidized to oxidized LDL. When LDL, especially oxidized LDL (ox-LDL), is in excess, the cholesterol it carries can build up in the walls of arteries, causing atherosclerosis over time. Therefore, LDL is called the “bad cholesterol”. Low density lipoprotein is positively correlated with the occurrence of coronary heart disease (CHD) and atherosclerotic damage, which is an important index for lipid disease classification and risk prediction. Low Density Lipoprotein Cholesterol (LDL-C) Assay Kit provides a convenient tool for detection of LDL-C. The principle is that LDL-C in serum was separated with precipitant, and cholesterol esterase was used to catalyze the hydrolysis of cholesterol ester into free cholesterol (FC) and free fatty acid (FFA), so as to convert cholesterol ester into FC. Furthermore, cholesterol oxidase catalyzed FC oxidation to produce △4-cholestenone and H2O2. Then peroxidase catalyzes the oxidation of 4-amino-antipyrine and phenol with H2O2 to produce red quinone compounds has a characteristic absorption peak at 500 nm.