Principle of the Procedure

Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Total Antioxidant Capacity (TAC) Assay Kit provides a convenient tool for detection of Total Antioxidant Capacity (TAC) in serum, plasma, animal and plant tissues, cells, bacteria, urine and other biological fluids. The principle is that in an acidic environment, antioxidants can reduce Fe3+-Tripyridine triazine (Fe3+-TPTZ) to produce blue Fe2+-TPTZ, and the total antioxidant capacity of samples was obtained by determining the content of Fe2+-TPTZ at 593 nm.