Principle of the Procedure

Antioxidants play an important role in preventing the formation and scavenging of free radicals and other potentially toxic oxidizing species. Total antioxidant status (TAS) refers to the ability of antioxidants in the antioxidant system to scavenge free radicals and produce antioxidant effects. Under normal circumstances, the body’s antioxidant system constantly scavenges excessive free radicals, so that the body’s total antioxidant capacity and free radical production balance. The total antioxidant capacity is reduced, which means that the body’s ability to scavenge free radicals is weakened. The measurement of total antioxidant capacity can inform the body’s ability to balance free radicals, which is conducive to mastering the body’s defense ability. Therefore, it is very important to measure the total antioxidant capacity in the body. This Kit provides a convenient tool for detection of Total Antioxidant Capacity in serum, plasma, animal and plant tissues, cells, bacteria, urine and other biological fluids. The principle is that in an acidic environment, antioxidants can reduce Fe3+-Tripyridine triazine (Fe3+-TPTZ) to produce blue Fe2+-TPTZ, and the total antioxidant capacity of samples was obtained by determining the content of Fe2+-TPTZ at 593 nm.